PROCESSES AND MATERIALS FOR CARRYING OUT MICROCHEMICAL AND MICROBIOLOGICAL TESTS

摘要

A microbiological culture test process for detecting the presence of tryptophan-deaminase-positive microorganisms, which comprises (a) culturing a microorganism to be tested in the presence of L-tryptophan in an effective amount and for a period sufficient to allow production of indolepyruvic acid by the organism if it is of tryptophan-deaminase-positive character, (b) exposing the culture to a chromogenic amount of metal ions which are chromogenic with indolepyruvate, and to a fluorophor, and (c) assessing the fluoroscence of the culture after steps (a) and (b), thereby to show a reduction in fluoroscence in the presence of a tryptophan-deaminase-positive microorganism.