发明名称 |
PREPARING ANTIBODIES FROM CHO CELL CULTURES FOR CONJUGATION |
摘要 |
The invention is based in part on the observation that a CHO cell oxidizing enzyme, particularly QSOX1, can survive a seemingly rigorous antibody purification process to reduce subsequent conjugation efficiency of the antibody to a drug. Whether the oxidizing enzyme survives the purification procedure depends on which purification techniques are employed which can vary from one antibody to another. With knowledge that contamination with a CHO cell oxidizing enzyme is a potential problem for subsequent conjugation, a suitable purification scheme can be devised for any antibody that eliminates or at least reduces CHO oxidizing enzyme(s) to an acceptable level. |
申请公布号 |
EP3074036(A1) |
申请公布日期 |
2016.10.05 |
申请号 |
EP20140863650 |
申请日期 |
2014.11.21 |
申请人 |
SEATTLE GENETICS, INC. |
发明人 |
HAYES, BRADLEY;BEAM, KEVIN;MEYER, DAMON;LYON, ROBERT;VALLIERE-DOUGLASS, JOHN |
分类号 |
A61K39/395;A61K47/48;C07K1/14 |
主分类号 |
A61K39/395 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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