发明名称 |
GENERAL ENZYME ASSAY WHERE ENZYME CONVERTS BLOCKED INHIBITOR TO ACTIVE INHIBITOR OF SECOND ENZYME |
摘要 |
A method for assay for an unknown enzyme suspected to be present in a liquid includes signal amplification by use of a second enzyme and a blocked modulator for the second enzyme. Unknown enzyme in the liquid removes a blocking group from the blocked modulator. The resulting modulator activates or inhibits the second enzyme which catalyzes an indicator reaction in which a substrate is converted to a product. The presence or absence of the unknown enzyme in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the indicator reaction. The concentration of the enzyme in the sample may be determined by the measurement of the signal. The invention includes a kit of materials useful for performing the method of the invention. |
申请公布号 |
AU3940889(A) |
申请公布日期 |
1990.02.15 |
申请号 |
AU19890039408 |
申请日期 |
1989.08.08 |
申请人 |
BECTON, DICKINSON AND COMPANY |
发明人 |
THOMAS H. SCHULTE |
分类号 |
C12Q1/00;A61B5/00;C12Q1/34;C12Q1/44 |
主分类号 |
C12Q1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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