发明名称 |
PROTEIN COMPLEXES HAVING FACTOR VIII:C ACTIVITY AND PRODUCTION THEREOF |
摘要 |
2068728 9107490 PCTABS00005 Recombinant protein complexes having human Factor VIII:C activity are expressed in a eukaryotic host cell by transforming the host cell with first and second expression cassettes encoding a first polypeptide substantially homologous to human Factor VIII:C A domain and a second polypeptide substantially homologous to human Factor VIII:C C domain, respectively. In the present invention, the first polypeptide may be extended having at its C-terminal a human Factor VIII:C B domain N-terminal peptide, a polypeptide spacer of 3-40 amino acids, and a human Factor VIII:C B domain C-terminal peptide. Expression of the second polypeptide is improved by employing an .alpha.1-antitrypsin signal sequence.
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申请公布号 |
CA2068728(A1) |
申请公布日期 |
1991.05.18 |
申请号 |
CA19902068728 |
申请日期 |
1990.11.15 |
申请人 |
NOVO NORDISK A/S;CHIRON CORPORATION |
发明人 |
CHAPMAN, BARBARA;BURKE, RAE L.;RASMUSSEN, MIRELLA E.;MIKKELSEN, JAN M. |
分类号 |
C12N15/09;A61K38/00;A61K38/43;A61P7/00;A61P7/02;C07H21/04;C07K;C07K7/06;C07K14/00;C07K14/745;C07K14/755;C07K14/81;C07K16/00;C07K19/00;C12N;C12N5/10;C12N15/12;C12N15/62;C12N15/85;C12P;C12P21/02;C12R1/91;(IPC1-7):C12N15/62;A61K38/37 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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