发明名称 |
Substrate composition, immunoassay and urea determination employing immobilized urease. |
摘要 |
<p>A method for enzyme immunoassay of a ligand includes urease as a label. A bound fraction of ligand and antiligand conjugated to urease is formed on a solid support. The urease component of the bound fraction is contacted with a substrate composition for urease which includes a compound converted to ammonia by the urease, a tetrazolium salt and a pH dependent reducing agent which reduces the tetrazolium salt when the pH of the assay medium has been raised by the ammonia. The tetrazolium salt is reduced to a colored insoluble formazan which precipitates as a detectable spot on the support. The invention includes the substrate composition and a kit of materials for performing the assay.</p> |
申请公布号 |
EP0458231(A1) |
申请公布日期 |
1991.11.27 |
申请号 |
EP19910108115 |
申请日期 |
1991.05.18 |
申请人 |
BECTON DICKINSON AND COMPANY |
发明人 |
STEWART, THOMAS N.;VONK, GLENN P.;MAPES, JAMES P. |
分类号 |
C12Q1/58;G01N33/535;G01N33/543;G01N33/58 |
主分类号 |
C12Q1/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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