发明名称 |
DIRECT MOLECULAR CLONING OF A MODIFIED EUKARYOTIC CYTOPLASMIC DNA VIRUS GENOME |
摘要 |
A method is disclosed for producing a modified eukaryotic cytoplasmic DNA virus by direct molecular cloning of a modified DNA molecule comprising a modified cytoplasmic DNA virus genome. The inventive method comprises the steps of (I) modifying under extracellular conditions a DNA molecule comprising a first cytoplasmic DNA virus genome to produce a modified DNA molecule comprising the modified cytoplasmic DNA virus genome; (II) introducing the modified DNA molecule into a first host cell which packages the modified DNA molecule into infectious virions; and (III) recovering from the host cell virions comprised of the modified viral genome. The host cell is infected with a helper virus which is expressed to package the modified viral genome into infectious virions. Examples of packaging a modified poxvirus genome by a helper poxvirus of the same or different genus are described. Also disclosed are novel poxvirus vectors for direct molecular cloning of open reading frames into a restriction enzyme cleavage site that is unique in the vector. In one model poxvirus vector, the open reading frame is transcribed by a promoter located in the vector DNA upstream of a multiple cloning site comprised of several unique cleavage sites.
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申请公布号 |
CA2076839(A1) |
申请公布日期 |
1993.02.27 |
申请号 |
CA19922076839 |
申请日期 |
1992.08.25 |
申请人 |
IMMUNO AKTIENGESELLSCHAFT |
发明人 |
DORNER, FRIEDRICH;SCHEIFLINGER, FRIEDRICH;FALKNER, FALKO G.;PFLEIDERER, MICHAEL |
分类号 |
C12N7/00;A61K39/275;A61P31/12;A61P37/04;C07K14/16;C07K14/745;C07K14/755;C12N7/01;C12N7/08;C12N9/64;C12N9/68;C12N9/74;C12N15/00;C12N15/09;C12N15/64;C12N15/863;(IPC1-7):C12N15/86;C12N15/12;C12N15/49;C12N15/55;C12N15/57;C12N15/63 |
主分类号 |
C12N7/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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