TRANSFORMING GROWTH FACTOR-E

摘要

A substantially pure epithelial growth factor (TGFe) and a method for purification are provided. Bovine TGFe is a monomeric glycoprotein of Mr 22,000 - 25,000, as determined by SDS-PAGE under nonreducing conditions, and is characterized by the N-terminal amino acid sequence N-Asp-Val-Lys-Pro-Asp-Met-Glu-Val-Ser-Pro-Pro-Asp-Tyr-Thr (SEQ ID NO:1) and internal peptide sequences of Pro-Glu-Pro-Lys-Lys-Pro-Glu (SEQ ID NO:2) and Xaa-Gly-Leu-Ala-Ala-Ala-Gly-Pro-Ala-Pro-Ser-Glu-Ser-Gln-Glu-Lys-Lys-Pr o-Leu-Lys-Pro-Glu-Gly-Ala, where Xaa is Ala or Pro (SEQ ID NO:48). The characteristic biological activity of TGFe includes stimulation of soft agar colony formation of SW-13 cells, and stimulation of A431 cells, epithelial cells, fibroblasts, epidermal keratinocytes and certain transformed cell lines.