| 摘要 |
A pulsatile perfusion bioreactor for culturing one or more engineered blood vessels having a lumen and a wall is provided. The bioreactor includes a chamber for holding the engineered blood vessel and cell culture media; a mechanical property monitoring system for measuring axial tensile stress and strain, circumferential tensile stress and strain, and/or shear stress imparted on the vessel wall; and a pump system for delivering cell culture media through the vessel lumen, wherein the vessel is exposed to a composite pressure waveform and a composite flow waveform as the media is delivered there through. The pump system includes a steady flow and peristaltic pumps. Further, the composite pressure and flow waveforms each include a mean component, a fundamental frequency component, and a second harmonic frequency component. The bioreactor also includes a computer interface for monitoring and adjusting the composite waveforms to maintain a predetermined stress levels. |
| 主权项 |
1. A pulsatile perfusion bioreactor for culturing one or more engineered blood vessels having a lumen and a wall, the pulsatile perfusion bioreactor comprising:
a chamber for holding the engineered blood vessel and cell culture media; a mechanical property monitoring system for measuring and controlling axial tensile stress, circumferential tensile stress, shear stress, or a combination thereof imparted on the wall of the engineered blood vessel and for measuring and controlling axial stretch, circumferential stretch, or a combination thereof imparted on the wall of the engineered blood vessel; a pump system for delivering cell culture media through the lumen of the engineered blood vessel, wherein the engineered blood vessel is exposed to a composite pressure waveform and a composite flow waveform as the cell culture media is delivered through the lumen, the pump system comprising a steady flow pump and a peristaltic pump, wherein the composite pressure waveform comprises a mean pressure component, a first harmonic frequency pressure component, and a second harmonic frequency pressure component, and wherein the composite flow waveform component comprises a mean flow component, a first harmonic frequency flow component, and a second harmonic frequency flow component; and a computer interface for monitoring and adjusting the composite pressure waveform, the composite flow waveform, or a combination thereof to maintain a predetermined axial tensile stress level, a predetermined circumferential stress level, a predetermined shear stress level, or a combination thereof. |
