Combination of growth factors, cytokines, antibacterial/antiviral factors, stem cell stimulating factors, complement proteins C3A/C4A, and chemotactic factors

摘要

The present invention refers to a combination of growth factors, cytokines, antibacterial/antiviral factors, stem cell stimulating factors, complement proteins C3a/C4a, and chemotactic factors. The invention also relates to a process for the preparation of said combination from serum, placenta or colostrum and to composition containing said combination for use in the treatment of conditions requiring tissue repair and regeneration and for the substitution of stem cell therapies.

主权项

1. A pharmaceutical composition comprising a biomaterial and a combination of cytokines, growth factors, chemotactic factors, stem cell stimulating factors, antibacterial and/or antiviral factors and complement proteins, wherein the combination comprises all the components listed in the following tables:
cytokinesMinimalMaximalConcentrationConcentration(pg cytokine/mg(pg cytokine/mgcombination)combination)Interleukin-1a0.802.90Interleukin-1b0.020.09Interleukin-21.0010.00Interleukin-40.040.17Interleukin-61.005.00Interleukin-80.100.70Interleukin-90.507.00Interleukin-100.502.80Interleukin-121.502.10Interleukin-151.0010.00Interleukin-1720.00150.00Interferon-gamma5.0050.00Tumor necrosis factor α30.0070.00Interleukin-1 Receptor Agonist10.0030.00Sum71.46340.76 and growth factors:MinimalMaximalConcentrationConcentration(pg growth(pg growthfactor/mgfactor/mgcombination)combination)Transforming growth_factor-β1100.00500.00Insulin-like growth factor-11000.005000.00Nerve growth factor2.005.00Platelet-derived growth factor10.0050.00Epidermal growth factor4.809.40Bone morphogenetic protein15.0050.00Fibroblast growth factor basic100.00500.00Fibroblast growth factor-25.0020.00Hepatocyte growth factor50.00100.00Vascular endothelial growth factor35.00260.00Sum1321.806494.40 and stem cell stimulating factors:MinimalMaximalConcentrationConcentration(pg stem cell(pg stem cellstimulating stimulating factor/mgfactor/mgcombination)combination)Granulocyte colony-10.0050.00stimulating factorGranulocyte-macrophage 100.00750.00colony-stimulating factorLeukemia inhibitory factor50.00200.00Stem cell factor1.005.00Stromal derived factor-130.00100.00Sum191.001105.00 and chemotactic factors:MinimalMaximalConcentrationConcentration(pg chemotactic (pg chemotacticfactor/mgfactor/mgcombination)combination)EOTAXIN-1, EOTAXIN-2, 5.0020.00and/or EOTAXIN-3Monocyte chemotactic factor-11.004.00Sum6.0024.00 and antibacterial and/or antiviral factors:MinimalMaximalConcentrationConcentration(μg antibacterial(μg antibacterialand/or antiviral and/or antiviralfactors/mgfactors/mgcombination)combination)Transferrin2.0020.00Lactoferrin1.005.00Lysozyme5.0020.00Lactoperoxydase10.0030.00Sum of the above antibacterial18.0075.00and/or antiviral factors and complement proteins:MinimalMaximalConcentrationConcentration(pg complement(pg complementprotein/mgprotein/mgcombination)combination)Complement C3A0.200.70Complement C4A0.902.00Sum1.102.70 wherein the biomaterial is selected from the group consisting of: collagen, hyaluronic acid, a gelatinous protein mixture secreted by Engelbreth-Holm-Swarm mouse sarcoma cells, hydrocolloids, polylactides, polyglycolides, and polycaprolactones.