| 摘要 |
PCT No. PCT/SE95/01319 Sec. 371 Date May 7, 1997 Sec. 102(e) Date May 7, 1997 PCT Filed Nov. 7, 1995 PCT Pub. No. WO96/14406 PCT Pub. Date May 17, 1996A method of preparing a nucleic acid sequence capable of hybridizing to a target DNA sequence without requiring knowledge of this DNA sequence comprises the steps of ligating or otherwise linking a DNA fragment related to the target DNA sequence to a nucleotide sequence containing the recognition motif of an asymmetrically cleaving restriction enzyme, and subjecting the construct to the restriction enzyme to thereby cleave the construct within the target-related DNA fragment part thereof. A vector therefor comprises a site for insertion of the target-related DNA fragment, and a recognition sequence for an asymmetrically cleaving restriction enzyme or enzymes on one or both sides of said insertion site. |
