METHOD OF PURIFYING HYDROPHOBIC POLYPEPTIDE

摘要

A method of purifying a hydrophobic polypeptide by high-performance liquid chromatography using a mobile phase comprising a solvent mixture wherein the content of trifluoroacetic acid is 3-10 % (v/v) and a packing material comprising polyvinyl alcohol. This method is useful because a lung surfactant prepared from the hydrophobic polypeptide purified by this method has a surface activity superior to that of the hydrophobic polypeptide purified by the conventional methods.