Evaluation peptide for use in quantification of protein using mass spectrometer, artificial standard protein, and method for quantifying protein

摘要

Disclosed is an evaluation peptide for evaluating the efficiency of a pretreatment in the quantification of a protein using a mass spectrometer, having high reliability and high general versatility. Also disclosed is an artificial standard protein comprising the evaluation peptide. Further disclosed is a method for quantifying a protein utilizing the artificial standard protein. Specifically disclosed is a method for selecting a peptide which consists of an amino acid sequence not agreeing with that in a naturally occurring protein and a variant thereof and capable of being detected by mass spectrometry and which has an amino acid that can be recognized by a protein-digesting enzyme, and using the peptide as an evaluation peptide for use in the quantification of a protein by a mass spectrometer.

主权项

1. An evaluation peptide for quantification of a protein using a mass spectrometer, the peptide being composed of any of the amino acid sequences shown in the following (1) to (4):(1) VGAPGVPALK;(SEQ ID NO: 1)(2) QIGDPTVPSGVK;(SEQ ID NO: 2)(3) DAPGSGLK;(SEQ ID NO: 3)and(4) NVAPAGPTLK.(SEQ ID NO: 4)