Methods of polypeptide identification, and compositions therefor

摘要

Methods are disclosed for identifying one or more proteins or polypeptides comprised by a sample. The methods comprise determining binding of each polypeptide with respect to each binding pool of a plurality of binding pools, wherein each binding pool comprises one or more probes which bind a structure comprised by a protein or polypeptide. In some aspects, polypeptides can be denatured and separated into individual polypeptide strands and immobilized on a solid support prior to determining binding of the binding pools. A protein, polypeptide or polypeptide strand can be identified by searching, in at least one database, for a protein or polypeptide sequence comprising binding pool targets either identical to or most similar to the binding pool targets comprised by the protein, polypeptide or polypeptide strand to be identified. Kits for identifying proteins, polypeptides and polypeptide strands are also disclosed.

主权项

1. A method of identifying a protein or polypeptide strand in a sample, the method comprising:
immobilizing, on a solid support, a plurality of protein or polypeptide strands comprised by a sample, such that the protein or polypeptide strand to be identified is separated by an optically resolvable distance from other protein or polypeptide strands comprised by the sample; determining presence or absence, in the protein or polypeptide strand to be identified, of each binding pool target of a plurality of binding pool targets, wherein each binding pool target comprises one or more binding targets for one or more probes comprised by a binding pool wherein each binding pool comprises one or more probes, and binds a binding pool target comprised by a population of proteins or polypeptides described in a database; contacting the protein or polypeptide strand to be identified sequentially or simultaneously with each binding pool; for each binding pool, detecting binding of at least one probe comprised by the binding pool to at least one binding target if the at least one binding target is comprised by the protein or polypeptide strand to be identified; assigning a digital signature to the protein or polypeptide strand to be identified which represents the presence or absence of binding pool targets comprised by the protein or polypeptide strand to be identified; and identifying, in at least one database, a protein or polypeptide having a digital signature with the shortest Hamming distance to that of the protein or polypeptide strand to be identified.