Vaccine and serum for endotoxin associated disease immunization and treatment, detoxified endotoxin, and bacterial mutant

摘要

A combination vaccine, with methods of preparation and treatment, for protection against Gram negative bacterial diseases. The vaccine includes a killed suspension of a bacterial mutant from the taxonomic family Enterobacteriaceae, a B-lymphocyte proliferating immune modulator, and a protein and lipid binding adjuvant. A hyperimmune serum for treating diseased animals is prepared by vaccinating a donor animal with the combination vaccine and then preparing clot serum containing antigen specific antibodies. The mutant is ATCC No. 53000 and is produced by ionizing radiation of Salmonella enteritidis. The immune modulator is a detoxified extract of lipopolysaccharide and is useful with many antigens to enhance primary immune response.