发明名称 |
Method for chromosomes classification |
摘要 |
A method for finding L internal reference vectors for classification of L chromosomes or portions of chromosomes of a cell, the L chromosomes or portions of chromosomes being painted with K different fluorophores or combinations thereof, wherein K basic chromosomes or portions of chromosomes of the L chromosomes or portions of chromosomes are each painted with only one of the K different fluorophores, whereas the other L-K of the L chromosomes or portions of chromosomes are each painted with a different combination of the K different fluorophores, the method comprising the steps of (a) using a multi-band collection device for measuring a first vector for each pixel of each of the L chromosomes or portions of chromosomes; (b) identifying pixels belonging to each of the K basic chromosomes or portions of chromosomes and defining the pixels as basic pixels, so as to obtain K basic classes of basic pixels; (c) using at least one basic pixel from each of the K basic classes for obtaining K basic vectors, the K basic vectors being K internal reference vectors; (d) using the K basic vectors for identifying pixels belonging to the other L-K chromosomes or portions of chromosomes; and (e) using the pixels belonging to the other L-K chromosomes or portions of chromosomes for calculating the other L-K internal reference vectors, thereby finding all of the L internal reference vectors. A method for classification of L chromosomes or portions of chromosomes of a cell similarly painted using the above method for finding L internal reference vectors, and using the L reference vectors for classification of each of the pixels into one of L classification classes. And, images presenting color chromosomes.
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申请公布号 |
US5798262(A) |
申请公布日期 |
1998.08.25 |
申请号 |
US19970844516 |
申请日期 |
1997.04.18 |
申请人 |
APPLIED SPECTRAL IMAGING LTD. |
发明人 |
GARINI, YUVAL;KATZIR, NIR;WINE, DAVID;CABIB, DARIO |
分类号 |
G01N33/48;C12M1/00;C12M3/00;C12N15/09;C12N15/11;C12Q1/68;G01J3/02;G01J3/12;G01J3/26;G01J3/28;G01J3/44;G01J3/453;G01N1/28;G01N21/64;G01N21/75;G01N33/483;G01N33/50;G01N33/52;G01N33/569;G01N33/58;G01N37/00;G02B21/00;G02B21/16;G06K9/00;G06K9/76;(IPC1-7):C12M1/34;G06F15/00 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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