发明名称 |
Oxidized phospholipid degrading enzyme and gene thereof |
摘要 |
An oxidized phospholipid degrading enzyme is provided, which plays an important role in the oxygen stress preventive mechanism in organisms. The enzyme hydrolyzes a 1-acyl-2- omega -carboxyfatty acid acyl-3-phosphatidylcholine as a substrate at the 2-ester bond thereof to form 1-acyl-2-lyso-3-phosphatidylcholine. The activity of the enzyme is slightly enhanced by 4 mM calcium chloride. The molecular mass of the enzyme is 95+/-5 kDa (by gel filtration). The enzyme is composed of three subunits whose molecular masses have been found to be 29 kDa, 30 kDa and 45 kDa, respectively, by SDS-polyacrylamide electrophoresis. A gene coding the enzyme is also provided. This gene is important for the synthesis of the enzyme by genetic engineering.
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申请公布号 |
US5849557(A) |
申请公布日期 |
1998.12.15 |
申请号 |
US19940283917 |
申请日期 |
1994.08.03 |
申请人 |
SUNTORY LIMITED |
发明人 |
ADACHI, HIDEKI;TSUJIMOTO, MASAFUMI;INOUE, KEIZO;ARAI, HIROYUKI |
分类号 |
C12N9/16;C12N9/18;C12N15/09;C12N15/53;(IPC1-7):C12N9/20 |
主分类号 |
C12N9/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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