METHOD FOR PREDICTING ORGAN TRANSPLANT REJECTION USING NEXT-GENERATION SEQUENCING

摘要

The present invention relates to a method for non-invasively predicting an organ transplant rejection by measuring the ratio between an organ-donor-specific nucleic acid sequence and a recipient-specific nucleic acid sequence in a biological sample of an organ transplant recipient and, more specifically, to a method for predicting an organ transplant rejection by measuring the ratio of a donor-derived marker sequence and a recipient-derived marker sequence regarding three or more markers selected in Tables 1 to 10 by testing a biological sample (e.g. blood) of an organ transplant recipient and predicting the organ transplant rejection based on the measurement. The method for predicting an organ transplant rejection using next-generation sequencing (NGS) according to the present invention or a digital base amplification technique can apply even to a very small amount of sample, is faster and inexpensive, is fast in the rate of data analysis, can apply to all organ transplants of all races in the word, and can simultaneously detect error probability of a sequence analysis, and thus is useful to non-invasively predict the organ transplant rejection.