发明名称 |
ALPHAVIRUS VECTORS FOR PARAMYXOVIRUS VACCINES |
摘要 |
A DNA vector comprises a first DNA sequence which is complementary to at least part of an alphavirus RNA genome and having the complement of complete alphavirus DNA genome replication regions, and a second DNA sequence encoding a paramyxovirus protein, particularly a respiratory syncytial virus fusion (RSV F) protein or a RSV F protein fragment that generates antibodies that specifically react with RSV F protein, the first and second DNA sequences being under the transcriptional control of a promoter, preferably a cytomegalovirus promoter, which may include Intron A. Such vectors also contain a further nucleotide sequence located between the promoter sequence and the alphavirus sequence to enhance the immunoprotective ability of the RSV F protein when expressed in vivo. Such DNA vectors may be used to immunize a host against disease caused by infection with RSV or other paramyxovirus, including a human host, by administration thereto, and may be formulated as immunogenic compositions with pharmaceutically-acceptable carriers for such purposes. Such vectors also may be used to produce antibodies for detection of RSV or other paramyxovirus infection in a sample.
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申请公布号 |
WO9925858(A1) |
申请公布日期 |
1999.05.27 |
申请号 |
WO1998CA01064 |
申请日期 |
1998.11.13 |
申请人 |
CONNAUGHT LABORATORIES LIMITED;PARRINGTON, MARK;LI, XIAOMAO |
发明人 |
PARRINGTON, MARK;LI, XIAOMAO |
分类号 |
C12N15/09;A61K31/711;A61K39/155;A61P31/12;C07K14/115;C07K14/135;C12N15/45;C12N15/86;(IPC1-7):C12N15/86;A61K31/70 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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