Novel bioactivating substance

摘要

A bioactivating substance extracted and manufactured from activated tissues, showing positive color reactions to amino acids, saccharides, phosphorus and silicic acid and showing negative qualitative reactions to proteins and phenols, comprises silicon components in an amount of more than 20 mu g calculated as silicon per mg of dried substance. A bioactivating substance extracted and manufactured from activated tissues, showing positive color reactions to: (a) amino acids (by a ninhydrin reaction); (b) saccharides (by an orcinol-iron (III) chloride-hydrochloric acid method; (c) phosphorus (by a molybdenum blue method); and (d) silicic acid (by a molybdenum blue method); and showing negative qualitative reactions to: (a) proteins (by a trichloroacetic acid method); and (b) phenols (by a ferric chloride method); comprises silicon components in an amount of more than 20 mu g calculated as silicon per mg of dried substance. ACTIVITY : Anti-allergic. MECHANISM OF ACTION : Histamine inhibition; hyaluronidase inhibitor. The effect of test compounds on histamine liberation from Wistar rat mast cells was determined by means of generation of fluorescence using o-phthalaldehyde. The suppressing rate to histamine liberation by addition of the tested compound was expressed in terms of %. The suppressing rate to histamine liberation at various silicon compounds in the test samples was as follows: 16.9% at 100 mu g/ml, 43.4% at 300 mu g/ml, 53% at 400 mu g/ml and 65.1% at 500 mu g/ml. The results showed that the bioactivating substance had a high antihistamine activity.