发明名称 |
Transfection, storage and transfer of male germ cells for generation of transgenic species and genetic therapies |
摘要 |
A composition for in vivo transfection of non-human mammalian male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into non-human mammalian male germ cells comprises the administration of the composition to a non-human mammalian. A method for isolating or selecting transfected cells utilizes a reporter gene, and a method for administering transfected male germ cells utilizes male germ cells which have been transfected in vitro.
|
申请公布号 |
US6316692(B1) |
申请公布日期 |
2001.11.13 |
申请号 |
US19980191920 |
申请日期 |
1998.11.13 |
申请人 |
READHEAD CAROL W.;WINSTON ROBERT |
发明人 |
READHEAD CAROL W.;WINSTON ROBERT |
分类号 |
A01K67/027;A61K35/12;A61K35/48;A61K45/00;A61K48/00;A61P15/08;A61P43/00;C12N5/076;C12N5/10;C12N15/09;C12N15/85;C12Q1/68;(IPC1-7):C12N15/09;C12N15/00;C12N15/63;A01N43/04;A61K31/70 |
主分类号 |
A01K67/027 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|