发明名称 |
Monitoring hybridization during PCR |
摘要 |
<p>A method for detecting a target nucleic acid sequence in a biological sample during amplification comprising adding a thermostable polymerase and primers configured for amplification of the target nucleic acid to the biological sample, amplifying the target nucleic acid sequence by PCR in the presence of a heat labile double-strand specific dye, conducting the PCR through a number of amplification cycles, illuminating the biological sample comprising the amplified target nucleic acid sequence with light at a wavelength absorbed by the dye, and detecting a fluorescent emission from the dye related to the quantity of the amplified target nucleic acid sequence in the sample.</p> |
申请公布号 |
EP1179600(A1) |
申请公布日期 |
2002.02.13 |
申请号 |
EP20010203467 |
申请日期 |
1997.06.04 |
申请人 |
UNIVERSITY OF UTAH RESEARCH FOUNDATION |
发明人 |
WITTWER, CARL T.;RIRIE, KIRK M.;RASMUSSEN, RANDY P. |
分类号 |
C12N15/09;B01L3/14;B01L7/00;C12Q1/68;G01N21/03;G01N21/07;G01N21/25;G01N21/64;G01N33/00;G01N35/00;G01N35/02;(IPC1-7):C12Q1/68;G01N33/58 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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