发明名称 Use of HPPD inhibitors as selcetion agents in plant transformation
摘要 The present invention relates to a method for transforming plant cells by introducing a heterologous gene into said plant cells with a gene for tolerance to HPPD inhibitors as a selection marker, said method comprising the steps of: a) preparing and culturing competent plant cells capable of receiving the heterologous gene in a suitable medium, b) transforming the competent cells with the heterologous gene and the selection marker, c) growing and selecting the transformed cells comprising the heterologous gene in a suitable medium, characterized in that a step for bleaching the competent plant cells is carried out before the transformation step (b), by introducing a suitable amount of HPPD inhibitor into the suitable culture medium of the competent plant cells.
申请公布号 US2002100076(A1) 申请公布日期 2002.07.25
申请号 US20010921263 申请日期 2001.08.02
申请人 GARCON FREDERIC;PELISSIER BERNARD 发明人 GARCON FREDERIC;PELISSIER BERNARD
分类号 C12N15/82;(IPC1-7):A01H5/00 主分类号 C12N15/82
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