Method for the manufacture of DNA

摘要

The present invention relates to a method for manufacturing DNA comprising the steps of preparing n single stranded base-DNA-oligonucleotides which form immediately consecutive parts of the nucleotide sequence of the DNA being manufactured, in which the second to the n-th base-DNA-oligonucleotide is phosphorylated at the 5' end and n is at least 2; preparing at least (n-1) single stranded joint-DNA-oligonucleotides capable of functioning as ligation templates for the base-DNA-oligonucleotides; contacting the base-DNA-oligonucleotides with the joint-DNA-oligonucleotides; subjecting the resultant product DNA-hybrid to a ligation reaction; and finally subjecting the resultant reaction product to an exonuclease reaction, in which the DNA strand of the reaction product formed by ligated base-DNA-oligonucleotides includes at least two cap-structures. Further the invention relates to DNA obtained by the method and a kit for carrying out the method.