| 摘要 |
FIELD: biotechnology, microbiology, amino acids. SUBSTANCE: invention relates to method for preparing amino acids by fermentation using microorganisms of genus E. coli as producers of amino acids. The strain of microorganism E. coli constructed on the basis the strain E. coli unable to utilize sucrose by insertion of genes encoding phosphoenolpyruvate-dependent sucrose-6-phosphotransferase system (sucrose PTC) or genes encoding the transport system in symport with proton (permease of type lac Y), invertase, fructokinase and sucrose-specific repressor (sucrose non-PTS) is cultured in nutrient medium and amino acid is isolated from cultural fluid. The strain E. coli VL 2055 comprising genes of sucrose non- PTS is a producer of threonine. The strain E. coli NZ10 rht A23/pAL4 comprising genes of sucrose PTS is a producer of homoserine. The strain E. coli 44-3-15 comprising genes of sucrose non-PTS is a producer of isoleucine. The strain E. coli VL 612 comprising genes of sucrose PTS is a producer of lysine. The strain E. coli VL 613 (VKPM B-3423) is a producer of lysine. The strain E. coli VL 1971 comprising genes of sucrose PTS is a producer of valine. The strain E. coli SV 164/pGH5 comprising genes of sucrose non-PTS is a producer of tryptophane. Invention provides the enhancement of expression of amino acids. EFFECT: valuable properties of strain. 13 cl, 4 dwg, 7 tbl, 8 ex |
