CELLULAR LINE OF IMMORTALIZED KERATINOCYTES AND MELANOCYTES (VARIANTS), METHOD FOR IMMORTALIZATION OF HUMAN SKIN CELLS FOR PREPARING IMMORTALIZED KERATINOCYTES, METHOD FOR IMMORTALIZATION OF HUMAN SKIN CELLS FOR PREPARING IMMORTALIZED MELANOCYTES, SERUM-FREE MEDIUM (VARIANTS), ANALYSIS OF CUTANEOUS RESPONSES

摘要

FIELD: biotechnology. SUBSTANCE: cellular lines of human immortalized keratinocytes DK2-NR, Dk3-NR, FK2-NR and immortalized melanocytes DM2-NR are obtained by skin cells sowing on serum-free medium NR-2. Then keratinocytes are transferred to selective medium NR-3 and melanocytes to medium NR-4. Keratinocytes and melanocytes are infected with retroviral construction and then immortalized keratinocytes are grown in proliferative medium NR-2 and differentiated in medium containing 1.5 mM of calcium and immortalized melanocytes are proliferated in medium M2. Immortalized keratinocytes and melanocytes are used for analysis of cutaneous responses. Invention can be used for preparing immortalized cells of human cells. EFFECT: improved preparing method. 12 cl, 2 dwg, 10 tbl