| 摘要 |
PROBLEM TO BE SOLVED: To increase yield of insoluble polypeptides as inclusion bodies after expression of recombination. SOLUTION: A method for recombinant production of a desired polypeptide comprising a step expressing a nucleic acid encoding polypeptide in a microbial host cell, a step forming inclusion bodies containing the polypeptide in cytoplasm of the host cell, and a step isolating, solubilizing and naturing the polypeptide, characterized in that after fermentation the host cell or the host cell content is incubated at a temperature of 40°C or higher for at least 10 minutes and subsequently insoluble polypeptide is isolated from the host cell, this method providing an improved yield in inclusion bodies containing the desired polypeptide. COPYRIGHT: (C)2005,JPO&NCIPI
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