Fast thermo-optical particle characterisation

摘要

The present invention relates to a method and an apparatus for a fast thermo-optical characterization of particles. In particular, the present invention relates to a method and a device to measure the stability of (bio)molecules, the interaction of molecules, in particular biomolecules, with, e.g. further (bio)molecules, particularly modified (bio)molecules, particles, beads, and/or the determination of the length/size (e.g. hydrodynamic radius) of individual (bio)molecules, particles, beads and/or the determination of length/size (e.g. hydrodynamic radius).

主权项

1. A method for measuring inter- and/or intra-molecular interactions of particles in a solution comprising:
(a) providing a sample with marked particles in a solution; (b) exciting fluorescently the marked particles and detecting a first fluorescence of the excited particles; (c) irradiating a laser light beam into the solution to obtain a spatial temperature distribution in the solution around the irradiated laser light beam; (d) detecting a second fluorescence of the particles in the solution at a predetermined time after irradiation of the laser into the solution has been started, and (e) characterizing the inter- and/or intra-molecular interactions of the particles based on the first and second detections, wherein the sample with marked particles in a solution is in a chamber in a multi-well plate.