Determination of serum anti-mullerian hormone as a diagnostic test for spay in companion animals

摘要

This invention relates to non-surgical assays that can be used to determine whether an animal that has a non-postpartum anestrous period of three months or greater, such as a dog or a cat, has been spayed. In particular, the present invention relates to devices, kits and methods that allow correlation of levels of Anti-Müllerian Hormone with the state of being spayed.

主权项

1. A method of assaying serum samples from a female cat or dog for the presence of Anti-Müllerian Hormone (AMH) comprising:
contacting a serum sample obtained from a female cat or dog that is not reproductively senescent and that has a non-postpartum anestrous period or interestrous of three months or greater with an antibody that binds to Anti-Müllerian Hormone (AMH) to form an antibody-Anti-Müllerian Hormone complex; contacting said antibody-Anti-Müllerian Hormone complex with a labeled detection reagent to from a labeled detection reagent-antibody-Anti-Müllerian Hormone complex; and detecting the presence of said labeled detection reagent-antibody-Anti-Müllerian Hormone complex in an enzyme-linked immunosorbant assay or lateral flow assay.