METHOD FOR DETERMINING THE CELL AGGRESSIVENESS GRADE OF CANCER CELLS OR OF CANCER STEM CELLS

摘要

Method for determining, in vitro, the cell aggressiveness grade of cancer cells or for detecting cancer stem cells in a cell sample originating from a solid tissue suspected of being cancerous, includes: a) dissociating the cell cluster constituting the sample into a suspension of whole and viable isolated cells, b) macroscopically sorting the cells to obtain homogeneous subpopulations, c) calibrating at least one microwave electromagnetic sensor resonating at its own resonance frequency, d) presenting the dissociated and sorted cells to the calibrated sensor, e) interrogating the sensor and determining its new resonance frequency having received the cells, f) calculating the variation in overall dielectric permittivity of the cells according to the variation in working frequency, which constitutes the electromagnetic signature of the cells. The macroscopic sorting is without prior labelling and is based on the intrinsic properties of the cells. A kit for implementing the method is also described.

主权项

1. Process for determining in vitro the cell aggressiveness grade of cancer cells or for detecting cancer stem cells in a cell sample originating from solid tissue that is suspected of being cancerous, comprising at least the following steps:
a. Dissociation of the cell cluster constituting the sample into a suspension of whole and viable isolated cells, b. Macroscopic sorting of cells to obtain homogeneous subpopulations, c. Calibration of at least one microwave electromagnetic sensor resonating at its own resonance frequency, d. Presentation of the cells that are dissociated and sorted according to steps a. and b. on the at least one previously calibrated sensor, e. Interrogation of the at least one sensor and determination of the new resonance frequency of said at least one sensor having received the cells, f. Calculation of the variation in overall dielectric permittivity of the cells based on the variation of the work frequency, which constitutes the electromagnetic signature of the cells.