Engineered transaminase polypeptides

摘要

The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.

主权项

1. An engineered transaminase enzyme, wherein said transaminase enzyme comprises a polypeptide sequence comprising the substitution N45H in SEQ ID NO: 2 and having transaminase activity with increased thermostability at 50° C., as compared to the polypeptide of SEQ ID NO: 2.