| 摘要 |
PURPOSE:To obtain accurate measurements easily even in the case of specimen containing high-concentration antigen, in the nephelometric immuno-assay method for immunological measurement, by comparing the intensity of scattered lights measured at different angles. CONSTITUTION:A primary light 34 from a light source 32 is caused to enter a specimen 36 which contains antigen of unknown concentration in a cell 35, and scattered lights at different angles thereof 37, 38 (angle difference of more than 5 degrees; preferably, two sets of angle 30 to 45 degrees and angle 80 to 100 degrees) are respectively detected in photo detectors 39, 40, and amplified. Then, recording in a recorder 45, the intensities of both outputs are compared. The intensities of scattered lights with respect to the scattered light measuring angles alpha, beta are expressed in the function formula (1) in which the antigen concentraion C is a variable, respectively, and two values of antigen concentrations are obtained each. As the true antigen concentration, CXalpha and CXbeta or the mean of the two is taken when formula (2) is established, and CXalpha', CXbeta', or the mean of the two is taken when it is not established.
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