| 摘要 |
<p>PURPOSE:To prepare L-lysine, in high yield, by culturing L-lysine-producing bacteria belonging to Brevibacterium genus and sensitive to fluoropyruvic acid. CONSTITUTION:L-lysine-producing bactera belonging to Brevibacterium genus or Corynebacterium genus, are irradiated with UV radiation or treated with a mutagenic agent such as N-methyl-N'-nitro-N-nitrosoguanidine, etc. to obtain variant sensitive to fluoropyruvic acid. The variant is separated from the original bacteria by replica process, etc., and cultured in an ordinary nutrient medium at 24-37 deg.C and pH5-9 for 2-7 days under aeration. L-lysine can be separated from the medium by ion exchange resin treatment, etc., in high yield.</p> |
