| 摘要 |
A fluorimetric detector comprises a radionuclide source e.g. a <s63>sNi or <s147>sPm source, as the fluorescence excitation source; and when adapted for use with flowing liquid systems, especially liquid chromatography systems, comprises a passageway for liquid flow and a monitoring system for monitoring fluorescent radiation, in which the monitoring system is located outside the liquid passageway the walls of which are transparent at least in part. Also a method for detection and determination of a species, either a fluorescent species or a species which quenches fluorescence, by fluorescence measurements comprises exposing liquid comprising the species to fluorescence exciting radiation from a radionuclide source, monitoring fluorescence thereby produced and comparing this with the fluorescence of the same liquid in the absence of the species. The use of a radionuclide as the fluorescence exciting source overcomes problems of instability of excitation of prior art visible radiation excitation fluorescence detectors. |
