ANTIBODY AGAINST ACID PHOSPHATASE ISOENZYMES FOR DETECTION OF PROSTATIC CANCER

摘要

An immunochemical method for detecting prostatic acid phosphatases has been developed which is useful in clinical laboratory testing for prostate cancer and in enzyme and protein determinations. An isoenzymatically homogeneous prostatic acid phosphatase antigen is prepared by isolating purified acid phosphatase from cancerous human prostate tissue. The antigen stimulates the production of diagnostic antibodies which are highly specific, do not cross-react with other acid phosphatase isoenzymes and can be used in countercurrent immunoelectrophoresis (showing a sensitivity of 0.4 I.U. of enzyme activity or 20 ng/ml enzyme protein) and in a solid phase fluorescent immunoassay (showing a sensitivity of 60 pg/ml enzyme protein with excitation of the alpha-napthol hydrolysis product at 340 nm and emission at 465 nm) with no false positives and few false negatives in diagnosing cancer of the prostate.