FACILITY FOR COUPLING A BIOREACTOR WITH A DEVICE FOR PHYSICOCHEMICALLY ANALYSING OR COLLECTING SAMPLES

摘要

The invention concerns a facility for coupling a bioreactor for culturing bio-organisms, in dynamic mode, with at least one device for physicochemically analyzing a fluid from this bioreactor or collecting samples of this fluid, the device being equipped with a pump and an injector, the bioreactor being equipped with means for supplying culture medium and xenobiotic(s). The facility is remarkable in that the bioreactor is connected to a closed perfusion loop provided with a pump, fitted with a sampling loop and in that the facility comprises:
a device for injecting a cleaning product into said sampling loop,two six-way valves, the first valve being connected to the closed perfusion loop, to the sampling loop and to a cleaning loop, the second valve being connected to the cleaning loop and an injection loop that connects the injector to at least one of the devices.

主权项

1. A coupling facility of a bioreactor for culturing bio-organisms, in dynamic mode, with at least one unit for physicochemical analysis of fluid coming from this bioreactor or for collecting samples of this fluid, said unit being equipped with a pump and an injector, the bioreactor being equipped with means of supplying culture medium and xenobiotic(s), wherein the bioreactor is connected to a closed perfusion loop provided with a pump, this perfusion loop being fitted with a derivation loop, so-called “sample loop” and in that the facility comprises:
an injection device of a cleaning product into said sample loop, a first valve comprising six ways, and a second valve comprising six ways,the first valve being connected to the closed perfusion loop, to the sample loop and to a cleaning loop, the second valve being connected to the cleaning loop and to an injection loop which connects the injector to at least one of the units for physicochemical analysis or collecting samples.