| 摘要 |
<p>PCT No. PCT/DK82/00082 Sec. 371 Date Apr. 11, 1983 Sec. 102(e) Date Apr. 11, 1983 PCT Filed Sep. 14, 1982 PCT Pub. No. WO83/01074 PCT Pub. Date Mar. 31, 1983.Human insulin or B-30 esters thereof are prepared by reacting an insulin derivative of the formula <IMAGE> (I) wherein R is hydroxyl or an amino acid radical being different from threonine, and -A- and -B- represent the A- and B-chains with the same amino acid sequence as in human insulin, with a carboxyl protected and optionally hydroxyl protected L-threonine derivative in a concentration of from 2 to about 6 moles per liter in the presence of trypsin or a trypsin-like enzyme in a reaction medium containing water and optionally also an organic cosolvent at a pH value of from 5 to 9 and at a temperature below 50 DEG C., followed, if desired, by removal of any protecting groups present. Human insulin is prepared in an easy and simple way in high yield and in high purity.</p> |
