发明名称 |
ES Cell-Derived Mice From Diploid Host Embryo Injection |
摘要 |
Genetically modified mice and nucleic acid constructs for making the genetically modified mice are described. A first mouse having a gene encoding an activator (such as a Cre recombinase) operably linked to a developmentally-regulated promoter (such as a Nanog promoter) is provided. A second mouse having a toxic responder gene (such as a gene encoding diphtheria toxin A) is provided, where the toxic gene is expressed only in the presence of an activator. Embryos from a mating of the first and the second mouse are provided as host embryos suitable for generating mice from donor cells introduced into the host embryos. Ablating the ICM of a mouse embryo physically, chemically, or genetically is described, as well as making FO generation mice that are substantially or in full derived from donor cells, employing a host mouse embryo with an ablated or nonproliferating ICM. |
申请公布号 |
US2016316728(A1) |
申请公布日期 |
2016.11.03 |
申请号 |
US201615207281 |
申请日期 |
2016.07.11 |
申请人 |
REGENERON PHARMACEUTICALS, INC. |
发明人 |
Poueymirou William;DeChiara Thomas M.;Auerbach Wojtek;Economides Aris N.;Gale Nicholas W.;Frendewey David;Valenzuela David M. |
分类号 |
A01K67/027;C12N15/90;C12N5/16;C12N15/85 |
主分类号 |
A01K67/027 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
Tarrytown NY US |