摘要 |
PROBLEM TO BE SOLVED: To provide engineered zinc finger proteins that target 5-enolpyruvyl sikimate-3-phosphate synthase (EPSPS) genes in plant, and methods for use such zinc finger proteins in modulating gene expression, gene inactivation, and target gene modification.SOLUTION: The invention provides a non natural zinc finger protein (ZFP) that binds to an EPSPS target genomic region comprising one or plural engineered zinc finger binding domains, a polynucleotide encoding such a ZFP and a donor vector, where the donor vector comprises a first and second DNA sequences where (i) the first sequence is homologous to a third sequence, and the second sequence is homologous to a fourth sequence; and (ii) the third and fourth sequences are chromosomal EPSPS DNA sequences.SELECTED DRAWING: Figure 14E |