Device method of making, separating, and purifying artepillin C in propolis

摘要

A method of making, separating, and purifying artepillin C in propolis includes mixing propolis with ethanol to obtain a propolis-ethanol extract; providing supercritical carbon dioxide and the propolis-ethanol extract to a chromatographic column to separate wax, artepillin C, and flavonoids; providing supercritical carbon dioxide, the artepillin C, and the flavonoids to an adsorption column to remove the flavonoids; keeping the adsorption column still; and providing ethanol to the adsorption column to obtain the purified artepillin C.

主权项

1. A method of obtaining artepillin C in propolis, comprising the steps of:
mixing propolis with ethanol to form a mixture, and then removing impurities of the mixture to obtain a propolis-ethanol extract; providing supercritical carbon dioxide and the propolis-ethanol extract to a chromatographic column to separate wax, artepillin C, and flavonoids from the propolis-ethanol extract, and then removing the wax at a bottom of the chromatographic column, and collecting the artepillin C and the flavonoids at a top of the chromatographic column; providing supercritical carbon dioxide, the artepillin C, and the flavonoids to an adsorption column, and then removing the flavonoids at a bottom of the adsorption column, wherein the artepillin C is adsorbed by the adsorption column; keeping the adsorption column still for a predetermined time; and providing ethanol to the adsorption column to separate the artepillin C from the adsorption column, and then collecting the artepillin C at a top of the adsorption column.