POLYDEOXYRIBONUCLEIC ACID HAVING GENE INFORMATION OF NOVEL PHYSIOLOGICALLY ACTIVE POLYPEPTIDE, REPLICABLE RECOMBINANT DNA CONTAINING SAID POLYDEOXYRIBONUCLEIC ACID, MICROORGANISM OR CELL TRANSFORMED BY SAID REPLICABLE RECOMBINANT DNA, NOVEL PHYSIOLOGICALLY ACTIVE POLYPEPTIDE, AND THEIR PREPARATION

摘要

PURPOSE:To provide an amino acid sequence of TNF (Tumor Necrosis Factor) deduced from the DNA sequence of TNF obtained by the DNA recombination technique. CONSTITUTION:The TNF polypeptide having the amino acid sequence of formula. TNF is a substance produced in the body of a rabbit by administering a substance activating the reticuloendothelial system to the rabbit, and stimulating with endotoxin, and is effective to kill only the tumor cell. The amino acid sequence of the TNF has been determined by the analysis of a serum TNF, and can be produced by introducing the recombinant DNA coding the amino acid sequence into a microbial cell, and culturing the cell.