Process for the sterile micropropagation of plant material

摘要

The invention relates to a process for the production of propagating material of plants by micropropagation in sterile tissue culture which comprises (a) planting sterile shoot cuttings of plants in solid nutrient medium in a suitable flask, filling up the flask with a suitable liquid nutrient medium optionally containing a growth regulator and growing the shoots in the submersed system; thereafter (b) cutting up into pieces the ramified culture thus obtained which contains a number of lateral buds and shoots, rooting the top-end shoots and planting the same after adaptation, and subjecting the cuttings prepared from the lower and middle parts of the shoot cultures to further cultivations according to step (a); and repeating steps (a) and (b) until the desired number of top-end shoots is reached.