摘要 |
<p>NEW MATERIAL:A compound of the formula (R<1>, R<2>, R<3> are H, protecting groups). USE:A reagent for elucidating the mechanism of resistance manifestation in a bacterium resisting antibiotics or a reagent for studying biotechnology and molecular biology. PREPARATION:For example, the benzyl ester of glycine which is the C-terminal aminoacid of this peptide is dissolved in DMF together with alanine whose amino group is protected with t-butoxycarbonyl group, p-toluenesulfonate salt and triethylamine. Then, a condensation reagent such as N,N'-dicyclohexyl- carbodiimide is added thereto under cooling to effect reaction. The amino- protecting groups are removed, then the operations are repeated in accordance with the aminoacid sequence of the peptide, further the protecting groups are eliminated as needed to give the compound of the formula.</p> |