FLUORESCENCE POLARIZATION ASSAY,REAGENTS,AND METHOD OF MAKING REAGENTS,FOR DETERMINATION OF DIGITOXIN

摘要

The present invention is directed to an improved fluorescent polarization assay for digitoxin, and to improved reagents for use in the assay. The reagent is formed as the reaction product of 3-amino- 3-deoxydigitoxigenin and 5- or 6-carboxyfluorescein, under conditions for forming amide linkages, to yield a tracer. The tracer forms a complex with antiserum specific to digitoxin, in proportion to the relative concentration of the tracer with respect to the concentration of the digitoxin present in the sample. It has been found that excellent results are obtained when such a reagent is used for measuring concentrations of digitoxin.