Production of reverse transcriptase

摘要

Methods are described for the production of the beta -subunit of reverse transcriptase using recombinant DNA techniques. Methods and compositions are described for cloning and expressing the gene coding for the beta -subunit of reverse transcriptase and characterizing and isolating the gene product. The recombinant plasmids constructed herein contain the necessary signals and controls for efficient expression of the reverse transcriptase gene in bacteria and other microorganisms. The isolated reverse transcriptase gene product is a functional, metabolically stable and biologically active protein.