Combined organ and hematopoietic cells for transplantation tolerance of HLA mismatched grafts

摘要

Methods and compositions are provided for combined transplantation of a solid organ and hematopoietic cells to an HLA mismatched recipient, where tolerance to the graft is established through development of a persistent mixed chimerism. An individual with persistent mixed chimerism, usually for a period of at least six months, is able to withdraw from the use of immunosuppressive drugs after a period of time sufficient to establish tolerance.

主权项

1. A method for transplantation of a HLA mismatched solid human organ, said method comprising:
implanting said HLA mismatched solid human organ in a recipient human body, wherein said HLA mismatched solid human organ is from a donor; treating said recipient with non-myeloablative conditioning; infusing said recipient with an engineered hematopoietic cell composition, said engineered hematopoietic cell composition comprising at least 1×107 CD34+ cells/kilogram recipient weight and at least 1×107 CD3+ T cells/kilogram recipient weight; and maintaining said recipient on an immunosuppressive regimen for a period of time sufficient to develop mixed chimerism for at least 6 months.