| 摘要 |
<p>Method for the estimation of an analyte which can be acylated which includes acylating the analyte with an acylcoenzyme A to liberate Co A, causing the Co A to react with 2-oxoglutarate in the presence of NAD and 2-oxoglutarate dehydrogenase to form NADH, and causing the NADH to reduce a further compound, the amount of reduction being measured. Preferably the acylation is an acetylation and the further compound is a tetrazolium salt which is reduced to a formazan. The method is suitable for the estimation of antibiotics which can be acetylated, e.g. thiamphenicol, chloramphenicol and gentamicin. A preferred tetrazolium salt is 2-(2'-benzothiazolyl)-5-styryl-3-(4'-phthalhydrazidyl)-tetrazolium chloride reduced in the presence of 1-methoxy-phenazinium methosulphate.</p> |
