| 摘要 |
A method for injecting a required liquid 3 into a culture vessel 2 to which adherent cells 1 are adhered, wherein the liquid injection method has a culture vessel tilting step for tilting the culture vessel 2 from a horizontal state within a range of a tilt angle (X °) of more than 0° to 50° around a horizontal axis 5, and a liquid injection step for injecting a liquid 3 at a predetermined linear velocity (Y mm/s) through a wall surface 2B of the culture vessel 2 tilted in the culture vessel tilting step, and the relationship of the tilt angle (X) and the linear velocity (Y) satisfies the formula: Ｙ ≦ ５．０７５Ｘ ＋ １２３. |
