| 摘要 |
Antibodies are used as biomarkers to assist in distinguishing gluten immune reactivity and sensitivity, silent celiac disease, Crohn's disease and other gut-related pathologies from classical celiac disease. In one class of embodiments, sera, saliva or other samples from a human or other animal are tested for antibodies to (a) a wheat antigen; (b) a gliadin antigen; and (c) one or more of a wheat germ agglutinin, a gluteomorphin, a glutenin, a deamidated glutenin, a prodynorphin, and a dynorphin. Test results are considered particularly interesting where the wheat antigen and the gliadin antigen are both selected from the group consisting of native and deamidated forms of α-gliadin 33-mer, α-gliadin-17-mer, γ-gliadin-15-mer, ω-gliadin-17-mer, and glutenin 21-mer. Test plates and kits can advantageously test for antibodies to at least three, five, seven or all of mixed wheat antigens, α-gliadin, γ-gliadin, ω-gliadin, glutenin, α-glutenin, wheat germ agglutinin, gluteomorphin, prodynorphins, transglutaminase-2, transglutaminase-3, transglutaminase-6, and gliadin-bound transglutaminase. |
| 主权项 |
1. A method of characterizing an antibody profile of an individual, comprising:
providing a first test surface comprising a coating of deamidated α-gliadin 33-mer (SEQ ID NO. 2), a second test surface comprising a coating of transglutaminase-2, and a third test surface comprising a coating of deamidated glutenin 21-mer (SEQ ID NO. 16); applying a sample from a human subject to the first test surface, the second test surface, and the third test surface; characterizing the formation of a complex between IgA immunoglobulins of the sample and the coating of the first test surface, the coating of the second test surface, and the coating of the third test surface; and recording values characteristic of the amount of complex formed between the IgA immunoglobulins and the coating of the first test surface, the coating of the second test surface, and the coating of the third test surface. |
