| 摘要 |
<p>PURPOSE:To obtain a human cell-culturing composition useful for cultivation of human lymphocyte in adoptive immunity remedy having excellent safety by preparing from human blood as a raw material not containing any human cell proliferation-inhibiting substance and microorganism, and containing a growth- and division-stimulating factor derived from human blood. CONSTITUTION:The objective human cell-culturing composition contains growth and division-stimulating factor without substantially containing any human cell proliferation-inhibiting substance and microorganism. Said microorganism is treated by adding a inactivator (preferably liquid ethylene oxide) into blood serum or blood plasma to be inactivated. Said human cell proliferation-inhibiting substance is various substances such as immunoglobulin, existing in the blood serum or blood plasma, giving an undersirable effect on or inhibiting proliferation of the human cell and is removed by salting out or a desalting treatment. Said human cell-culturing composition is advantageously used for cultivation of human lymphocyte in an adoptive immunity method. In said case, the human lymphocyte is cultured in a medium obtained by adding said human cell-culturing composition and lymphokine to a fundamental medium to proliferate and activate the human lymphocyte.</p> |
