Method of fabrication for capillary electrophoresis and electrochemical detector for the same

摘要

An improved electrochemical detector for use in capillary zone electrophoresis having a working electrode of the order of magnitude of 32 microns in diameter inserted into a separation capillary of the order of 50 microns in diameter is fabricated as follows. The separation capillary is bonded to a rigid collar and an electrical joint through a fracture is defined in the separation capillary. The collar is telescopically disposed within a first guide capillary approximately 1.2 millimeters outer diameter. The first guide capillary is then telescopically inserted into an outer sleeve capillary of approximately 1.25 millimeters in inner diameter. Similarly a working electrode is aspirated into a drawn down second guide tube of approximately 1.2 millimeters in outer diameter. A mercury pool is disposed in the second guide tube and a contacting wire is electrically coupled to the mercury pool and hence to the working electrode. The second guide tube is then disposed within the sleeve capillary with the two guide tubes moved toward each other so that the centralized working electrode is telescopically inserted within the separation capillary. The assembly is sealed and thus serves as a rugged electrochemical detector which can be integrally fabricated on the end of a separation column.